MyBioSource.com's HAMSTER ANTI MOUSE CD81 is a Hamster monoclonal antibody. The HAMSTER ANTI MOUSE CD81 Antibody was generated using CD81, and TSPAN28 as the antigen. It reacts with Mouse. This antibody has been shown to work in applications such as: ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blot.
Description
Hamster anti Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maecker et al. 2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding. Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006)